Determination of amphetamine and methamphetamine in umbilical cord

Determination of amphetamine and methamphetamine in umbilical cord using liquid chromatography-tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Nov 1;877(29):3701-6. Epub 2009 Sep 19.
Jones J, Rios R, Jones M, Lewis D, Plate C.
United States Drug Testing Laboratories, Des Plaines, IL 60018, United States. PMCID: PMC2782418 DOI: 10.1016/j.jchromb.2009.09.021

The use of meconium as a drug-screening matrix for newborns has been the gold standard of care for the past two decades. A recent study using matched pairs of meconium and umbilical cord demonstrated a high degree of agreement. The use of liquid chromatography-tandem mass spectrometry as a means to confirm amphetamines presumptive positive umbilical cord specimens for amphetamine and methamphetamine is described here for the first time. The limit of detection for both compounds was 0.2 ng/g. The limit of quantitation for both compounds was 0.6 ng/g. The assay was linear for both compounds up to 100 ng/g.